Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 General Information
Product name
Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1
Validated applications
ELISA
Specificity
Specifically recognizes the target-MMAE&MMAF.
Immunogen
MMAE
Source
Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
Purification
Protein A purified / Protein G purified
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. Contact us for customized product form or formulation.
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Recommended Usage
0.1-12.5 ng/mL
Note
MuseChem can customize this product per customer's request including product size, buffer components, etc.
Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 Images
Immobilized Mouse Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. AB00004) at 5 μg/mL, add Disitamab Vedotin (RC48) in the 50% Mouse serum and then add Biotinylated Human Her2, His,Avitag, premium grade at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (QC tested).
Immobilized ADC-MMAF at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. AB00004) with a linear range of 0.5-1.56 ng/mL (Routinely tested).
Immobilized Disitamab Vedotin (RC48) at 0.2 μg/mL (100 μL/well) can bind Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. AB00004) with a linear range of 0.1-2 ng/mL (Routinely tested).
ELISA binding of Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. AB00004) with Trastuzumab Deruxtecan, IgG1-MMAF, Sacituzumab Govitecam, Disitamab Vedotin (RC48), Trastuzumab MMAE and Trastuzumab-DM1 conjugated antibody respectively.
The coating antibody was Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. AB00004), used at 1 μg/mL concentration. The primary antibody were different payload conjugated antibodies, including Trastuzumab Deruxtecan, IgG1-MMAF, Sacituzumab Govitecam, Disitamab Vedotin (RC48), Trastuzumab MMAE and Trastuzumab-DM1 conjugated antibodies used at 0.5 μg/mL concentration. The secondary antibody was Peroxidase AffiniPure Goat Anti-Human IgG, Fcγ fragment specific (min X Bov, Hrs, Ms Sr Prot) used at 1:12000 concentration.
Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. AB00004) is specific to Disitamab Vedotin (RC48), Trastuzumab MMAE and IgG1-MMAF, and has no cross-reactivity with Trastuzumab Deruxtecan, Sacituzumab Govitecam and Trastuzumab-DM1 (Routinely tested).
