Phosphatidylinositol 4-kinase (PI4K), a membrane-associated PI kinase, plays a central role in cell signalling and trafficking.
An overview of PI4K
Phosphatidylinositol-4-kinase (PI4K), as the first important kinase in PI signaling pathway, can catalyze the phosphorylation of D4 on the inositol phospholipid (PI) ring to produce Phosphatidylinositol-4-phosphate (PI4P) in the process of phosphatidylinositol circulation and plays a central role in transmembrane signal transduction, as well as in the growth of multiple growth factors and hormone-regulated cells.
Major types of PI4K
PI4K can be divided into type II and III, of which type II includes alpha and beta subtypes (PI4kIIα and PI4kIIβ). The main function of PI4kIIα is closely related to the membrane transport associated with cis-golgi body. In addition, PI4kIIα plays an important role in neurotransmitter release, host cell activation and regulation of EGF.
Activation of PI4K
Studies have shown that many drugs and , phenothiazine, β-adrenergic stimulant, propranolol, hydrophilic cation, and poly L-lysine have a bidirectional effect on calf adrenal PIK that high concentration can inhibit enzyme activity while low concentration can activate enzyme activity. In addition, the heart toxin extracted from Cobra can increase the vitality of cell membrane PIK in rat leukaemia 2H3 cells and cultured canine kidney cells, which depends on the amount of time, temperature and venom. The synthetic apitoxin Mastoparan is a peptide compound with 40 amino acid residues. It can induce the release of granule and histamine in peritoneal mast cells of rats and can activate phospholipase C and phospholipid A2. Recently researches show that Mastoparan can significantly increase dog PIK and PIPK activity and the effect is dose and time dependent.
PI4K and diseases
The study of the function of phosphatidylinositol kinase found that the activity of enzyme in red blood cells such diseases as Alzheimer's disease, breast cancer and sickle-type anemia was significantly different from normal people. Therefore, the study on the regulation of PI4K on cell growth is not only important in cell biology, but also has important significance for pathology, which is closely concerned by people.
1. Li JM. (2009). Effect of PI4KIIα on angiogenesis induced by tumor by regulating HLF-Iα. Acta Biophysica Sinica.
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