Z-FR-AMC TFA

• CAT Number: I038689
• Molecular Formula: C35H37F3N6O8
• Molecular Weight: 726.7102
• Purity: 98%
• Solubility: To be determined
• Storage: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
• IUPAC Name: benzyl ((S)-1-(((S)-5-guanidino-1-((4-methyl-2-oxo-2H-chromen-7-yl)amino)-1-oxopentan-2-yl)amino)-1-oxo-3-phenylpropan-2-yl)carbamate 2,2,2-trifluoroacetate
• InChI: InChI=1S/C33H36N6O6.C2HF3O2/c1-21-17-29(40)45-28-19-24(14-15-25(21)28)37-30(41)26(13-8-16-36-32(34)35)38-31(42)27(18-22-9-4-2-5-10-22)39-33(43)44-20-23-11-6-3-7-12-23;3-2(4,5)1(6)7/h2-7,9-12,14-15,17,19,26-27H,8,13,16,18,20H2,1H3,(H,37,41)(H,38,42)(H,39,43)(H4,34,35,36);(H,6,7)/t26-,27-;/m0./s1
• InChIKey: AVPSADNFAQBJBZ-WMXJXTQLSA-N
• SMILES: CC(C1=CC=C(C=C1O2)NC([C@@H](NC([C@H](CC3=CC=CC=C3)NC(OCC4=CC=CC=C4)=O)=O)CCCNC(N)=N)=O)=CC2=O.OC(C(F)(F)F)=O

Z-FR-AMC is a fluorogenic substrate for cathepsin L. Upon enzymatic cleavage by cathepsin L, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify cathepsin L activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.

Reference

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