1. J Agric Food Chem. 2003 Feb 26;51(5):1162-8.
Analysis of strigolactones, germination stimulants for striga and orobanche, by
high-performance liquid chromatography/tandem mass spectrometry.
Sato D(1), Awad AA, Chae SH, Yokota T, Sugimoto Y, Takeuchi Y, Yoneyama K.
(1)Center for Research on Wild Plants, Utsunomiya University, Japan.
A simple and rapid analytical method for strigolactones, germination stimulants
for the root parasitic weeds witchweed (Striga spp.) and broomrape (Orobanche
spp.), has been developed using high-performance liquid chromatography connected
to tandem mass spectrometry (LC/MS/MS). The natural strigolactones (strigol,
sorgolactone, orobanchol, and alectrol) were clearly separated and identified by
LC/MS/MS. As low as 0.1 pg/microL of strigol and 0.5 pg/microL of sorgolactone
could be quantified, whereas 1 pg/microL was needed for the quantification of
orobanchol (S/N > 10). Using this method, it was found that red clover produces
orobanchol and alectrol but not strigol. The roots of red clover seedlings were
found to produce 13, 70, 58, and 65 pg of orobanchol/plant 1, 2, 3, and 4 weeks
after germination, respectively.
PMID: 12590450 [Indexed for MEDLINE]